Common sequences of Short DNA oligonucleotides will often be used as primers for sequencing cloned genes (Sanger sequencing). Choose between our generally utilised sequencing primers to initiate your sequence verification projects.
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In contrast to random primers, oligo (dT) primers aren't well suited for degraded samples but are exceptional for setting up cDNA libraries and suggested for use with reverse transcriptases (RTs) as Superscript IV RT.
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Enable you to can prefer to exclude sequences in the selected database from specificity examining if You aren't concerned about these.
This system will return, if at all possible, only primer pairs that don't generate a sound PCR products on unintended sequences and are therefore unique to your meant template. Note that the specificity is checked not simply for that ahead-reverse primer pair, and also for forward-ahead and also reverse-reverse primer pairs. Search manner
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Click "Incorporate a lot more organisms" label in order to restrict to various organisms (enter only one more info organism in each enter box). Entrez query (optional) Assist You need to use a daily entrez question to limit the databases try to find primer specificity. By way of example, enter a GenBank accession number to limit lookup to that individual sequence only (Caution: This implies the primer specificity will NOT be checked towards some other sequences except the specified a person). Primer specificity stringency
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